No sign of stalling for Cars film
The movie features the voices of Owen Wilson and Paul Newman and tells the tale of a talking vehicle by the name of Lightning McQueen.
It generated $31.2m (£16.9m), while another motoring-themed movie, The Fast and the Furious: Tokyo Drift entered at three, earning $24.1m (£13m).
It was beaten by Nacho Libre, with Jack Black playing a cook who also wrestles.
This made number two on the chart with takings of $27.5m (£14.8m).
Directed by John Lasseter, Cars will open in the UK on 28 July.
It is the seventh Disney-Pixar film and the first collaboration since Walt Disney acquired Pixar in January for $7.4bn (£4bn).
All of Pixar's other collaborations with the Disney studio have opened at the number one in North America.